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After the two lines had been isolated, seeds were sent to the Danforth Middle for additional molecular evaluation to determine why P34 was absent. Six similar genetic mutations were found in each, indicating the two lines may be related, Hymowitz stated. Related StoriesAllergic response can be unexpected clue in medical mysteryInternational research project examines effectiveness of tried and tested medicines for different diseasesReptile meals causes allergic reactions’The lack of the protein was verified by more-detailed two-dimensional protein assays,’ stated Eliot M. Herman, a lead scientist at Danforth who probed the seeds with post-doctoral researcher Monica A. Schmidt. ‘We after that isolated the gene in charge of the lesion, and we found there was an individual significant switch in the gene’s sequence that most likely produced a protein that could not be made as a stable item.’ Herman discovered P34 in the first 1990s and in 2003 had effectively used a gene-silencing technique to produce a soybean line in which P34 was ‘knocked out.’ However, because of public resistance to genetically modified products researchers have already been seeking a far more traditional approach.